Agrochemical residue analysis

The analysis of wine, juice and grapes by LC/MS/MS is accredited by the National Association of Testing Authorities (NATA) and is regularly exposed to international proficiency testing via FAPAS, BIPEA and NMI programs.

Multi-residue analysis – LC-MS/MS and GC-MS/MS

This multi-residue assay covers a broad range of fungicides and insecticides, targeting those agrochemicals which are currently registered, or recently deregistered, for viticultural use in the Australian wine industry. The analysis is available for grapes, juice and wine and uses the well-published QuEChERS extraction technique combined with the greater specificity of liquid chromatography with tandem mass spectrometry (LC-MS/MS) and gas chromatography with tandem mass spectrometry (GC-MS/MS). The LC-MS/MS assay can be performed alone or partnered with the GC-MS/MS assay to provide a greater coverage of agrochemical residues. The list of compounds determined in the LC-MS/MS assay is outlined below.

Ametoctradin Dimethoate Iodocarb Pydiflumetofen
Amisulbrom Dimethomorph Iprodione Pyraclostrobin
Atrazine Emamectin Malathion Pyrimethanil
Azinphos methyl Ethion Mandipropamid Pyriofenone
Azoxystrobin Etoxazole Mefentrifluconazole Quinoxyfen
Benalaxyl Fenamiphos Metalaxyl Simazine
Boscalid Fenarimol Methamidiphos Spinetoram
Buprofezin Fenhexamid Methidathion Spinosad
Captan Fenitrothion Methiocarb Spirotetramat
Carbaryl Fenpyrazamine Methomyl Spiroxamine
Carbendazim Fenpyroximate Methoxyfenozide Sulfoxaflor
Chlorantraniliprole Fenthion Myclobutanil Tebuconazole
Chlorpyrifos Fenvalerate Oxadixyl Tebufenozide
Chlorpyrifos-methyl Fluazinam Parathion-methyl Tetraconazole
Clothianidin Fludioxonil Penconazole THPI (see below)
Cyflufenamid Flumioxazin Pendimethalin Triadimefon
Cyproconazole Flusilazole Procymidone Triadimenol
Cyprodinil Fluxapyroxad Propiconazole Trichlorfon
Diazinon Hexaconazole Proquinazid Trifloxystrobin
Difenoconazole Indoxacarb Prothiofos

Compounds in BOLD type are not NATA-accredited

NOTE – THPI is only included in analysis of grapes NOT wine.

In addition to the multi-residue assay by LC-MS/MS, an extra six compounds can be analysed with the use of GC-MS/MS

Bifenthrin Chlorothalonil Cypermethrin Dicofol
THPI (wine only) Vinclozolin

Compounds in BOLD type are not NATA-accredited

Prices per analysis (excluding GST)
Analysis 1-7 samples 8+ samples
Multi-residue analysis – LC/MS/MS
Grapes/juice $231 $194.25
Wine $194.25 $157.50
Multi-residue analysis – LC/MS/MS and GC-MS/MS
Grapes/juice $250 $205
Wine $215 $175
Phenoxy acid herbicides (2,4-D, MCPA and triclopyr)
Grapes/juice/foliage $210 $175
Wine $195 $148
Target response time – 7-10 working days from receipt of samples
Volume required: wine or juice – 100 mL, grapes – 500g

The Uncertainty of Measurement for the agrochemical residue analyses is available here.

Prices per analysis (excluding GST)
Analysis (grapes or wine 2 samples
Total dithiocarbamates $245
Phosphorous acid $290
Glyphosate* $270
Target response time – 15 working days from receipt of samples
Volume required: wine or juice – 200 mL; grapes – 500g
Note: Above pricing is based on a minimum of two (2) samples.

*Glyphosate is determined as the sum of glyphosate, AMPA and n-acetyl glyphosate (as glyphosate) to an LoQ of 0.05 mg/kg

For information regarding agrochemicals for use in the wine industry or maximum residue limits for export destinations, please visit the AWRI’s agrochemicals webpage or download the booklet Agrochemicals registered for use in Australian viticulture (commonly known as the ‘Dog book’).

Drawing on the AWRI’s knowledge of maximum residue limits (MRLs) and impacts on winemaking, AWRI Commercial Services can advise wineries and growers on an appropriate course of action for the handling of samples with positive results.

For more information, please contact AWRI Commercial Services on (08) 8313 6600 or e-mail commercialservices@awri.com.au