Microbiological stability

Micro perusal

Samples submitted for perusal are filtered or centrifuged to isolate possible organisms. This material is viewed by microscopy to determine the presence of organisms and other amorphous material. The identity and viability of the microorganisms or any amorphous material observed is not determined.

Sterility check

For this analysis, 100 mL of wine is aseptically removed, filtered through 0.45 m membranes and incubated for 10 days on both WL media, and MRS media containing apple juice and cycloheximide. The plates are assessed after three days, seven and ten days. Results are taken at day ten and are reported as a pass or fail. Any colonies on the plates are described in terms of colour, size and morphology, and microscopy is used to determine the presence of yeast or bacteria. We do not accept any responsibility for sterility results that are requested for less than 10 days.

Microbial identification

We offer a Micro ID analysis that can identify thousands of different types of microbes including bacteria, fungi (yeasts) and molds. In conjunction with Microgenetix, the MicroSeq® system is used to identify bacteria and fungi (as extracted DNA) by sequencing specific genes. Following sequencing the results are compared to the MicroSeq® libraries and a ranked list of closest matches is provided. The bacterial library includes over 1700 species, and the fungal library includes over 1000 species.

Identification down to genus and usually species level can be done on samples submitted as slopes, colonies on plates, cultures, juice, ferments, and finished wine. Turn around times for slope and plate samples is 5 – 10 working days. Samples provided as cultures, juices, ferments or wines have a turn around time of 10 – 15 working days as they need to be plated out first to achieve single pure colonies.

Brettanomyces (plating)

For this analysis, 100 mL of wine is aseptically removed, filtered through 0.45 m membranes and incubated for 10 days on both WL media, and WL+C (containing cycloheximide) which prohibits the growth of non-Brettanomyces yeast. The plates are assessed after three days, seven and ten days. Results are taken at day ten and are reported as a presumptive pass (no growth) or presumptive fail (growth). Any colonies on the plates are described in terms of colour, size and morphology.

Prices per analysis
Analysis Per sample (excluding GST) Volume required Target response time
Micro perusal $55 750 mL bottle 24 hours
Sterility check $80 750 mL bottle 12 working days
Brettanomyces (plating) $60 750 mL bottle 12 working days
Micro identification $200 750 mL bottle 5-10 working days

** Important Safety Note **

Whenever samples are delivered for micro perusal or sterility analysis it is essential that any samples that are suspected to be unsafe are packed with a frozen cool pack, to limit pressure build-up resulting from uncontrolled fermentation.

Samples will be processed at the discretion of Commercial Services staff; any sample deemed to be unsafe may not be analysed.

For more information, please contact AWRI Commercial Services on (08) 8313 6600 or e-mail commercialservices@awri.com.au