New fluorescence method for detecting haze-forming proteins
he methods currently used for testing heat stability of white wine can be time-consuming, expensive and may not always accurately predict the amount of bentonite required to achieve protein stability. This could lead to over-fining, which might strip out phenolics and aroma compounds, or under-fining, which could increase the risk of protein instability. As an alternative, an efficient and rapid fluorescence-based method to detect haze-forming proteins in white wines has been developed at the AWRI, with the work recently published in the journal Food Chemistry.
The method uses a fluorescent compound that binds haze-forming proteins selectively. To validate it, a range of wines were analysed with results compared to an established HPLC method. A linear relationship was observed between the two methods over a range of relevant haze protein concentrations and the new method showed a low detection limit of 2 mg/L. The new technology can be deployed rapidly, without sample preparation, presenting an opportunity for routine testing. The full paper detailing this work is available from the AWRI library:
Mierczynska-Vasilev, A., Vasilev, A., Reilly, T., Bindon, K., Vasilev, K. 2022. Fluorescence sensing technology for the rapid detection of haze-forming proteins in white wine. Food Chem. 374: 131770.
For more information, contact Agnieszka Mierczynska-Vasilev on agnieszka.mierczynska-vasilev@awri.com.au. |
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The AWRI’s most recent publications are listed below.
2281 Coulter, A. Ask the AWRI: Interpreting AWRI smoke panel analysis results. Aust. N.Z. Grapegrower Winemaker (698): 42-43; 2022 (link to article).
2282 Kassara, S., Norton, E., Mierczynska-Vasilev, A., Lavi Sacks, G., Bindon, K.A. Quantification of protein by acid hydrolysis reveals higher than expected concentrations in red wines: implications for wine tannin concentration and colloidal stability. Food Chem. 385: 132658; 2022 (link to article).
2283 Cordente, A.G., Curtin, C.D., Solomon, M., Kulcsar, A.C., Watson, F., Pisaniello, L., Schmidt, S.A., Espinase Nandorfy, D. Modulation of volatile thiol release during fermentation of red musts by wine yeast. Processes 10(3): 502; 2022 (link to article).
2284 González-García, L.E., MacGregor, M.N., Visalakshan, R.M., Lazarian, A., Cavallaro, A.A., Morsbach, S., Mierczynska-Vasilev, A., Mailänder, V., Landfester, K., Vasilev K. Nanoparticles surface chemistry influence on protein corona composition and inflammatory responses. Nanomaterials 12(4): 682; 2022 (link to article).
2285 Day, M., Wilkes, E., Kirby, J., Wright, C., Krstic, M. Analytical approaches to wine authenticity. Wine Vitic. J. 38(2): 29-31; 2022 (link to article).
2286 Dry, P. Grenache blanc. Wine Vitic. J. 38(2): p. 58 ; 2022
2287 Habili, N., Little, A., Essling, M., Rinaldo, A. Grapevine leafroll-associated virus 3 and its management strategies in vineyards. Wine Vitic. J. 38(2): 34-40 ; 2022
2288 Ntuli, R., Saltman, Y., Ponangi, R., Jeffery, D., Bindon, K., Wilkinson, K. Applications of flash détente for red wine style differentiation. Wine Vitic. J. 38(2): 20-24 ; 2022
2289 Pisaniello, L., Watson, F., Siebert, T., Francis, L., Hixson, J.L. The varietal influence of flavour precursors from grape marc on monoterpene and C13-Norisoprenoid profiles in wine as determined by Membrane-Assisted Solvent Extraction (MASE) GC-MS. Molecules. 27(7): 2046; 2022 (link to article).
2290 Capone, D.L., Francis, I.L., Clingleffer, P.R. Evidence that methoxypyrazine accumulation is elevated in Shiraz rachis grown on Ramsey rootstock, increasing ‘green’ flavour in wine. Aust. J. Grape Wine Res. 28(2): 304-315; 2022 (link to article).
2291 Essling, M., Schwarz, C. Ask the AWRI: How to get the most out of copper sprays. Aust. N.Z. Grapegrower Winemaker (699): 76-78; 2022.
2292 Onetto, C.A., Sosnowski, M.R., Van den Heuvel, S., Borneman, A.R. Population genomics of the grapevine pathogen Eutypa lata reveals evidence for population expansion and intraspecific differences in secondary metabolite gene clusters. PLoS Genetics 18(4): e1010153; 2022 (link to article).
2293 Wang, Y.M., Ostendorf, B., Gautam, D., Habili, N., Pagay, V. Plant viral disease detection: from molecular diagnosis to optical sensing technology-a multidisciplinary review. Remote Sens. 14(7): 1542; 2022 (link to article). |